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Bio rad automated droplet generator12/27/2023 The AutoDG has a HEPA filter and maintains positive pressure, Reifsnyder explained. ![]() Carolyn Reifsnyder, a marketing manager at the digital biology center, said, "We're planning to start shipping at the end of September, so we'll take orders just before that. The company displayed photographs and video of the AutoDG at AACC. This detects fluorescence in droplets where amplification occurred, and provides absolute quantification of nucleic acid in a starting sample. Then the plate is loaded into Bio-Rad's droplet reader, the second generation of which is called the QX200. A plate full of droplets is sealed and transferred to a thermocycler. Droplet generation is performed in a separate, smaller device. With digital PCR, each droplet contains a PCR reaction. "This instrument will basically eliminate those differences." "Droplet digital PCR is sensitive enough to detect differences in people's pipetting habits," Patel said. The instrument may help reduce user-to-user variability seen with the current manual droplet generator. Viresh Patel, senior marketing manager at Bio-Rad's digital biology center, said he believes that the AutoDG is the first automated droplet generating system on any digital PCR platform. Unlike the current manual droplet generator, the automated system, called AutoDG, does not require a hood or clean room, and, after about five minutes of set-up time, generates 20,000 droplets per well in a 96-well plate format.Ĭompany representatives spoke to PCR Insider from the Bio-Rad booth on the floor of the American Association of Clinical Chemistry annual meeting and lab expo, held here this week. Advances in Clinical Genomics ProfilingĬHICAGO (GenomeWeb) - Bio-Rad has developed an automated droplet generator for its droplet digital PCR platform and plans to begin shipping the add-on in September.
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